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验证数据展示

  • WB analysis of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    WB analysis of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • IF Staining of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    IF Staining of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (67438-1-Ig, Clone: 1D4B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

PROX1 Monoclonal antibody, PBS Only

PROX1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67438-1-PBS

Print datasheet

CloneNo. 1D4B1

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 1D4B1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

67438-1-PBS targets PROX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen PROX1 fusion protein Ag9681 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name prospero homeobox 1
Synonyms PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 1D4B1
Calculated Molecular Weight 737 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC024201
Gene Symbol PROX1
Gene ID (NCBI) 5629
RRIDAB_2882674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92786
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.