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PROX1 Monoclonal antibody, PBS Only

PROX1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4B1

Cat no : 67438-1-PBS

Print datasheet

Synonyms

prospero homeobox 1, Prospero homeobox protein 1, PROX 1, PROX1

验证数据展示

  • WB analysis of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    WB analysis of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67438-1-Ig (PROX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    IHC staining of human lung cancer using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67438-1-Ig (PROX1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

  • IF Staining of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    IF Staining of HuH-7 using 67438-1-Ig (same clone as 67438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (67438-1-Ig, Clone: 1D4B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67438-1-PBS in a different storage buffer formulation.

产品信息

67438-1-PBS targets PROX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen PROX1 fusion protein Ag9681 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name prospero homeobox 1
Synonyms prospero homeobox 1, Prospero homeobox protein 1, PROX 1, PROX1
Calculated Molecular Weight 737 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC024201
Gene Symbol PROX1
Gene ID (NCBI) 5629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92786
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.