验证数据展示
经过测试的应用
| Positive WB detected in | HuH-7 cells, rat liver tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HuH-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67438-1-Ig targets PROX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | PROX1 fusion protein Ag9681 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | prospero homeobox 1 |
| 别名 | PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 1D4B1 |
| 计算分子量 | 737 aa, 83 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC024201 |
| 基因名称 | PROX1 |
| Gene ID (NCBI) | 5629 |
| RRID | AB_2882674 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92786 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PROX1 antibody 67438-1-Ig | Download protocol |
| IHC protocol for PROX1 antibody 67438-1-Ig | Download protocol |
| IF protocol for PROX1 antibody 67438-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
