PROX1 Monoclonal antibody
PROX1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1D4B1
验证数据展示
经过测试的应用
Positive WB detected in | HuH-7 cells, rat liver tissue |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HuH-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67438-1-Ig targets PROX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, rat, mouse |
Immunogen | PROX1 fusion protein Ag9681 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | prospero homeobox 1 |
Synonyms | prospero homeobox 1, Prospero homeobox protein 1, PROX 1, PROX1 |
Calculated Molecular Weight | 737 aa, 83 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC024201 |
Gene Symbol | PROX1 |
Gene ID (NCBI) | 5629 |
RRID | AB_2882674 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q92786 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PROX1 antibody 67438-1-Ig | Download protocol |
IHC protocol for PROX1 antibody 67438-1-Ig | Download protocol |
IF protocol for PROX1 antibody 67438-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Dev Cell Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells |