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CoraLite® Plus 488-conjugated PROX1 Monoclonal antibody

PROX1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D4B1

Cat No : CL488-67438

Print datasheet

Synonyms

prospero homeobox 1, Prospero homeobox protein 1, PROX 1, PROX1

验证数据展示

  • FC experiment of HepG2 using CL488-67438

    FC experiment of HepG2 using CL488-67438

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PROX1 (CL488-67438, Clone:1D4B1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HuH-7 using CL488-67438

    IF Staining of HuH-7 using CL488-67438

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using CoraLite® Plus 488 PROX1 antibody (CL488-67438, Clone: 1D4B1 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67438

    FC experiment of HepG2 using CL488-67438

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PROX1 (CL488-67438, Clone:1D4B1) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHuH-7 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67438-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67438 targets PROX1 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, rat, mouse
Immunogen PROX1 fusion protein Ag9681 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name prospero homeobox 1
Synonyms prospero homeobox 1, Prospero homeobox protein 1, PROX 1, PROX1
Calculated Molecular Weight 737 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC024201
Gene Symbol PROX1
Gene ID (NCBI) 5629
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92786
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PROX1 antibody CL488-67438Download protocol
FC protocol for CL Plus 488 PROX1 antibody CL488-67438Download protocol
Standard Protocols
Click here to view our Standard Protocols