验证数据展示
经过测试的应用
Positive IF/ICC detected in | HuH-7 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67438 targets PROX1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | PROX1 fusion protein Ag9681 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | prospero homeobox 1 |
别名 | PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 1D4B1 |
计算分子量 | 737 aa, 83 kDa |
观测分子量 | 90 kDa |
GenBank蛋白编号 | BC024201 |
基因名称 | PROX1 |
Gene ID (NCBI) | 5629 |
RRID | AB_2923773 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q92786 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 PROX1 antibody CL488-67438 | Download protocol |
FC protocol for CL Plus 488 PROX1 antibody CL488-67438 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |