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验证数据展示

  • WB analysis of K-562 using 24946-1-AP

    WB analysis of K-562 using 24946-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24946-1-AP (PRPF38A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24946-1-AP

    IF Staining of HepG2 using 24946-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24946-1-AP (PRPF38A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PRPF38A Polyclonal antibody

PRPF38A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24946-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RP5 965L7.1, Pre-mRNA-splicing factor 38A, Pre mRNA splicing factor 38A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PRPF38A Ready-To-Use IHC Kit. PRPF38A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24946-1-AP targets PRPF38A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PRPF38A fusion protein Ag20980 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
别名 RP5 965L7.1, Pre-mRNA-splicing factor 38A, Pre mRNA splicing factor 38A
计算分子量 312 aa, 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC000777
基因名称 PRPF38A
Gene ID (NCBI) 84950
RRIDAB_2879814
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NAV1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRPF38A was identified as an essential splicing factor in yeast and was found to be important for the progression of the splicing process to the first splicing step in this organism. PRPF38A is one of nine splicing factors that are specifically incorporated during the formation of the pre-catalytic spliceosomal B complex (B-specific proteins) and that are released again during spliceosome activation3 (PMID: 35869234). PRPF38A knockdown led to widespread intronic retention and altered splicing of transcripts of genes implicated in protein metabolism, mitosis, proteasome function and apoptosis (PMID: 28878028).

实验方案

Product Specific Protocols
WB protocol for PRPF38A antibody 24946-1-APDownload protocol
IF protocol for PRPF38A antibody 24946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols