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验证数据展示

  • IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 11171-1-AP (same clone as 11171-1-PBS)

    WB analysis of Jurkat using 11171-1-AP (same clone as 11171-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

    IP experiment of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

    IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 11171-1-AP (same clone as 11171-1-PBS)

    IHC staining of human stomach cancer using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

PRPF8 Polyclonal antibody, PBS Only

PRPF8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11171-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11171-1-PBS targets PRPF8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PRPF8 fusion protein Ag1652 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
别名
计算分子量 274 kDa
观测分子量 220 kDa
GenBank蛋白编号BC064370
基因名称 PRPF8
Gene ID (NCBI) 10594
RRIDAB_2171179
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P2Q9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.