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PRPF8 Polyclonal antibody, PBS Only

PRPF8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11171-1-PBS

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Synonyms

验证数据展示

  • IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 11171-1-AP (same clone as 11171-1-PBS)

    WB analysis of Jurkat using 11171-1-AP (same clone as 11171-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

    IP experiment of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

    IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 11171-1-AP (same clone as 11171-1-PBS)

    IHC staining of human stomach cancer using 11171-1-AP (same clone as 11171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

产品信息

11171-1-PBS targets PRPF8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen PRPF8 fusion protein Ag1652 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
Synonyms
Calculated Molecular Weight 274 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC064370
Gene Symbol PRPF8
Gene ID (NCBI) 10594
RRIDAB_2171179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P2Q9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.