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PRPSAP2 Polyclonal antibody

PRPSAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17814-1-AP

Print datasheet

Synonyms

PAP41, PRPSAP2

验证数据展示

  • WB analysis using 17814-1-AP

    WB analysis using 17814-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17814-1-AP (PRPSAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 17814-1-AP

    IHC staining of human pancreas using 17814-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17814-1-AP (PRPSAP2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of BxPC-3 using 17814-1-AP

    IF Staining of BxPC-3 using 17814-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PRPSAP2 antibody (17814-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inBxPC-3 cells, mouse brain tissue, HeLa cells, rat brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17814-1-AP targets PRPSAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRPSAP2 fusion protein Ag12132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoribosyl pyrophosphate synthetase-associated protein 2
Synonyms PAP41, PRPSAP2
Calculated Molecular Weight 369 aa, 41 kDa
Observed Molecular Weight 37-41 kDa
GenBank Accession NumberBC106050
Gene Symbol PRPSAP2
Gene ID (NCBI) 5636
RRIDAB_10598314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60256
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRPSAP2, also named as PAP41, is 369 amino acid protein, which Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis. PRPSAP2 is another protein involved in nucleic acid metabolism. The PRPSAP2 gene encodes part of the enzyme PRPP synthetase, which catalyzes the formation of phosphoribosylpyrophosphate, being a primary substrate for newly formed purine and pyrimidine nucleotides. Depletion of the PRPP synthetase causes growth arrest], the reverse has not been shown. Overexpression of SHMT1 and PRPSAP2 (and also COPS3) has been reported to occur in multiple myelomas . PRPSAP2 exists several isoforms with the range of molecular weight about 35-43 kDa.

实验方案

Product Specific Protocols
WB protocol for PRPSAP2 antibody 17814-1-APDownload protocol
IHC protocol for PRPSAP2 antibody 17814-1-APDownload protocol
IF protocol for PRPSAP2 antibody 17814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols