验证数据展示
经过测试的应用
| Positive IF/ICC detected in | BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-17814 targets PRPSAP2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PRPSAP2 fusion protein Ag12132 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoribosyl pyrophosphate synthetase-associated protein 2 |
| 别名 | PRPP synthase-associated protein 2, Phosphoribosyl pyrophosphate synthase-associated protein 2, PAP41, 41 kDa phosphoribosypyrophosphate synthetase-associated protein |
| 计算分子量 | 369 aa, 41 kDa |
| 观测分子量 | 37-41 kDa |
| GenBank蛋白编号 | BC106050 |
| 基因名称 | PRPSAP2 |
| Gene ID (NCBI) | 5636 |
| RRID | AB_3672682 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60256 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRPSAP2, also named as PAP41, is 369 amino acid protein, which Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis. PRPSAP2 is another protein involved in nucleic acid metabolism. The PRPSAP2 gene encodes part of the enzyme PRPP synthetase, which catalyzes the formation of phosphoribosylpyrophosphate, being a primary substrate for newly formed purine and pyrimidine nucleotides. Depletion of the PRPP synthetase causes growth arrest], the reverse has not been shown. Overexpression of SHMT1 and PRPSAP2 (and also COPS3) has been reported to occur in multiple myelomas . PRPSAP2 exists several isoforms with the range of molecular weight about 35-43 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PRPSAP2 antibody CL488-17814 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |