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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PRX5 Monoclonal antibody

PRX5 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B8C5

Cat no : CL488-67599

Print datasheet

Synonyms

Peroxiredoxin 5, PRDX5, B166, AOPP, AOEB166

验证数据展示

  • IF Staining of HeLa using CL488-67599

    IF Staining of HeLa using CL488-67599

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PRX5 antibody (CL488-67599, Clone: 1B8C5 ) at dilution of 1:600.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRDX5 Ready-To-Use IHC Kit. PRDX5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67599-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67599 targets PRX5 in IF/ICC applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, rat
Immunogen PRX5 fusion protein Ag12262 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 5
Synonyms Peroxiredoxin 5, PRDX5, B166, AOPP, AOEB166
Calculated Molecular Weight 214 aa, 22 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC110983
Gene Symbol PRX5
Gene ID (NCBI) 25824
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30044
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRX5 antibody CL488-67599Download protocol
Standard Protocols
Click here to view our Standard Protocols