PSAT1 Monoclonal antibody

PSAT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67619-1-Ig

产品说明书

CloneNo. 1B2C9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig and More (1)

应用

WB, IHC, IF/ICC, ELISA

EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67619-1-Ig targets PSAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, mouse, rat, pig
文献引用反应性human
免疫原 PSAT1 fusion protein Ag14076 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoserine aminotransferase 1
别名 EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1
计算分子量 370 aa, 40 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC001618
基因名称 PSAT1
Gene ID (NCBI) 29968
RRIDAB_2882821
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y617
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740 )

实验方案

Product Specific Protocols
WB protocol for PSAT1 antibody 67619-1-IgDownload protocol
IHC protocol for PSAT1 antibody 67619-1-IgDownload protocol
IF protocol for PSAT1 antibody 67619-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Thorac Cancer

Exosomal ERBB2IP contributes to tumor growth via elevating PSAT1 expression in non-small cell lung carcinoma

Authors - Xijuan Peng
humanIHC

Cancer Biother Radiopharm

Prognostic Signature in Osteosarcoma Based on Amino Acid Metabolism-Associated Genes

Authors - Liwen Feng
humanWB

Exp Mol Med

The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism

Authors - Su Hwan Park
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