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PSAT1 Monoclonal antibody, PBS Only

PSAT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2C9

Cat No : 67619-1-PBS

Print datasheet

Synonyms

EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1

验证数据展示

  • WB analysis using 67619-1-Ig (same clone as 67619-1-PBS)

    WB analysis using 67619-1-Ig (same clone as 67619-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67619-1-Ig (PSAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67619-1-Ig (same clone as 67619-1-PBS)

    IHC staining of human breast cancer using 67619-1-Ig (same clone as 67619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67619-1-Ig (PSAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 67619-1-Ig (same clone as 67619-1-PBS)

    IF Staining of HCT 116 using 67619-1-Ig (same clone as 67619-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSAT1 antibody (67619-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

产品信息

67619-1-PBS targets PSAT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen PSAT1 fusion protein Ag14076 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoserine aminotransferase 1
Synonyms EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1
Calculated Molecular Weight 370 aa, 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC001618
Gene Symbol PSAT1
Gene ID (NCBI) 29968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y617
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740 )