• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PSAT1 Polyclonal antibody

PSAT1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10501

Print datasheet

Synonyms

PSAT, PSA, Phosphoserine aminotransferase, EPIP, EC:2.6.1.52

验证数据展示

  • FC experiment of HepG2 using CL488-10501

    FC experiment of HepG2 using CL488-10501

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PSAT1 (CL488-10501) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10501-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10501 targets PSAT1 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen PSAT1 fusion protein Ag0776 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoserine aminotransferase 1
Synonyms PSAT, PSA, Phosphoserine aminotransferase, EPIP, EC:2.6.1.52
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC004863
Gene Symbol PSAT1
Gene ID (NCBI) 29968
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y617
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSAT1, also named PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PSAT1 antibody CL488-10501Download protocol
Standard Protocols
Click here to view our Standard Protocols