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PSD95-Specific,DLG4 Recombinant antibody, PBS Only

PSD95-Specific,DLG4 Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1I22

Cat No : 81106-1-PBS

Print datasheet

Synonyms

Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90

验证数据展示

  • WB analysis using 81106-1-RR (same clone as 81106-1-PBS)

    WB analysis using 81106-1-RR (same clone as 81106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    IF Staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSD95-Specific,DLG4 antibody (81106-1-RR, Clone: 1I22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

产品信息

81106-1-PBS targets PSD95-Specific,DLG4 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name discs, large homolog 4 (Drosophila)
Synonyms Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90
Calculated Molecular Weight 85 kDa
Observed Molecular Weight90-95 kDa
GenBank Accession NumberNM_001365
Gene Symbol DLG4
Gene ID (NCBI) 1742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78352
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).