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验证数据展示

  • WB analysis using 81106-1-RR (same clone as 81106-1-PBS)

    WB analysis using 81106-1-RR (same clone as 81106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    IF Staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSD95-Specific,DLG4 antibody (81106-1-RR, Clone: 1I22 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

PSD95-Specific,DLG4 Recombinant antibody, PBS Only

PSD95-Specific,DLG4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 81106-1-PBS

产品说明书

CloneNo. 1I22

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

81106-1-PBS targets PSD95-Specific,DLG4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 discs, large homolog 4 (Drosophila)
别名 Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90
计算分子量 85 kDa
观测分子量90-95 kDa
GenBank蛋白编号NM_001365
基因名称 PSD95
Gene ID (NCBI) 1742
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78352
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).