验证数据展示
经过测试的应用
Positive WB detected in | rat brain tissue, mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81106-1-RR targets PSD95-Specific,DLG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | discs, large homolog 4 (Drosophila) |
别名 | Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90 |
计算分子量 | 85 kDa |
观测分子量 | 90-95 kDa |
GenBank蛋白编号 | NM_001365 |
基因名称 | PSD95 |
Gene ID (NCBI) | 1742 |
RRID | AB_2923703 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P78352 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).
实验方案
Product Specific Protocols | |
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WB protocol for PSD95-Specific,DLG4 antibody 81106-1-RR | Download protocol |
IHC protocol for PSD95-Specific,DLG4 antibody 81106-1-RR | Download protocol |
IF protocol for PSD95-Specific,DLG4 antibody 81106-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |