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PSMA2 Monoclonal antibody

PSMA2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4A10

Cat no : 68240-1-Ig

Print datasheet

Synonyms

HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2

验证数据展示

  • WB analysis using 68240-1-Ig

    WB analysis using 68240-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68240-1-Ig (PSMA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 68240-1-Ig

    IHC staining of human cervical cancer using 68240-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68240-1-Ig (PSMA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA2 antibody included
  • IHC staining of human cervical cancer using 68240-1-Ig

    IHC staining of human cervical cancer using 68240-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68240-1-Ig (PSMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA2 antibody included

经过测试的应用

Positive WB detected inU2OS cells, A431 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMA2 Ready-To-Use IHC Kit. PSMA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68240-1-Ig targets PSMA2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, pig, rat
Immunogen PSMA2 fusion protein Ag5837 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, alpha type, 2
Synonyms HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC047697
Gene Symbol PSMA2
Gene ID (NCBI) 5683
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP25787
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

实验方案

Product Specific Protocols
WB protocol for PSMA2 antibody 68240-1-IgDownload protocol
IHC protocol for PSMA2 antibody 68240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols