PSMB1 Polyclonal antibody

PSMB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 11749-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Proteasome component C5, PMSB1, Macropain subunit C5, KIAA1838, HC5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells, human brain tissue, HepG2 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB1 Ready-To-Use IHC Kit. PSMB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11749-1-AP targets PSMB1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 PSMB1 fusion protein Ag2345 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 1
别名 Proteasome component C5, PMSB1, Macropain subunit C5, KIAA1838, HC5
计算分子量 241 aa, 26 kDa
观测分子量 25 kDa
GenBank蛋白编号BC020807
基因名称 PSMB1
Gene ID (NCBI) 5689
RRIDAB_2284481
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20618
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for PSMB1 antibody 11749-1-APDownload protocol
IHC protocol for PSMB1 antibody 11749-1-APDownload protocol
IF protocol for PSMB1 antibody 11749-1-APDownload protocol
FC protocol for PSMB1 antibody 11749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins.

Authors - Guiyou Tian
humanIF

Cancer Lett

Combinations of ivermectin with proteasome inhibitors induce synergistic lethality in multiple myeloma

Authors - Hongmei Luo
mouseWB,IHC

J Proteome Res

Novel breast cancer metastasis-associated proteins.

Authors - Ho Jiapei J
mouseWB

Front Pharmacol

Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.

Authors - Xin Xie
ratWB

Cardiovasc Drugs Ther

Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.

Authors - Kuixiang Chen
ratWB

PLoS One

Proteomic Analysis Reveals that Proteasome Subunit Beta 6 Is Involved in Hypoxia-Induced Pulmonary Vascular Remodeling in Rats.

Authors - Wang Jian J
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