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验证数据展示

  • IF Staining of U2OS using CL488-11749

    IF Staining of U2OS using CL488-11749

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 PSMB1 antibody (CL488-11749) at dilution of 1:200.

  • FC experiment of HeLa using CL488-11749

    FC experiment of HeLa using CL488-11749

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PSMB1 (CL488-11749) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated PSMB1 Polyclonal antibody

PSMB1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11749

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Proteasome component C5, PMSB1, Macropain subunit C5, KIAA1838, HC5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB1 Ready-To-Use IHC Kit. PSMB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11749-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11749 targets PSMB1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PSMB1 fusion protein Ag2345 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 1
别名 Proteasome component C5, PMSB1, Macropain subunit C5, KIAA1838, HC5
计算分子量 241 aa, 26 kDa
观测分子量 25 kDa
GenBank蛋白编号BC020807
基因名称 PSMB1
Gene ID (NCBI) 5689
RRIDAB_3083909
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20618
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PSMB1 antibody CL488-11749Download protocol
FC protocol for CL Plus 488 PSMB1 antibody CL488-11749Download protocol
Standard Protocols
Click here to view our Standard Protocols