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验证数据展示

  • WB analysis using 66403-1-Ig

    WB analysis using 66403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of Raji using 66403-1-Ig

    WB analysis of Raji using 66403-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66403-1-Ig

    IHC staining of human liver cancer using 66403-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB10 antibody included
  • IHC staining of human liver cancer using 66403-1-Ig

    IHC staining of human liver cancer using 66403-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB10 antibody included
  • WB analysis of rat thymus using 66403-1-Ig

    WB analysis of rat thymus using 66403-1-Ig

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 66403-1-Ig

    IHC staining of mouse kidney using 66403-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB10 antibody included
  • IF Staining of Jurkat using 66403-1-Ig

    IF Staining of Jurkat using 66403-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMB10 antibody (66403-1-Ig, Clone: 1G8C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

PSMB10 Monoclonal antibody

PSMB10 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66403-1-Ig

产品说明书

CloneNo. 1G8C1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

Multicatalytic endopeptidase complex subunit MECl-1, MECL1, Macropain subunit MECl-1, LMP10, EC:3.4.25.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Ramos cells, U-937 cells, rat thymus tissue, human spleen tissue, pig thymus tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PSMB10 Ready-To-Use IHC Kit. PSMB10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66403-1-Ig targets PSMB10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PSMB10 fusion protein Ag8835 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 10
别名 Multicatalytic endopeptidase complex subunit MECl-1, MECL1, Macropain subunit MECl-1, LMP10, EC:3.4.25.1
计算分子量 273 aa, 29 kDa
观测分子量 28 kDa
GenBank蛋白编号BC017198
基因名称 PSMB10
Gene ID (NCBI) 5699
RRIDAB_2881777
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40306
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMB10(Proteasome subunit beta type-10) is also named as LMP10, MECL1.It belongs to the peptidase T1B family and is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.

实验方案

Product Specific Protocols
WB protocol for PSMB10 antibody 66403-1-IgDownload protocol
IHC protocol for PSMB10 antibody 66403-1-IgDownload protocol
IF protocol for PSMB10 antibody 66403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols