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PSMB8 Monoclonal antibody, PBS Only

PSMB8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5B6

Cat no : 66759-1-PBS

Print datasheet

Synonyms

Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1

验证数据展示

  • IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB8 antibody included
  • WB analysis using 66759-1-Ig (same clone as 66759-1-PBS)

    WB analysis using 66759-1-Ig (same clone as 66759-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    IF Staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66759-1-Ig (same clone as 66759-1-PBS)

    IF Staining of HepG2 using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PSMB8 Ready-To-Use IHC Kit. PSMB8 primary antibody included.

产品信息

66759-1-PBS targets PSMB8 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PSMB8 fusion protein Ag6780 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Synonyms Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC001114
Gene Symbol PSMB8
Gene ID (NCBI) 5696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP28062
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).