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验证数据展示

  • IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    IHC staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMB8 antibody included
  • WB analysis using 66759-1-Ig (same clone as 66759-1-PBS)

    WB analysis using 66759-1-Ig (same clone as 66759-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    IF Staining of human lung cancer using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66759-1-Ig (same clone as 66759-1-PBS)

    IF Staining of HepG2 using 66759-1-Ig (same clone as 66759-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66759-1-PBS in a different storage buffer formulation.

PSMB8 Monoclonal antibody, PBS Only

PSMB8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66759-1-PBS

产品说明书

CloneNo. 2A5B6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSMB8 Ready-To-Use IHC Kit. PSMB8 primary antibody included.

产品信息

66759-1-PBS targets PSMB8 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMB8 fusion protein Ag6780 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
别名 Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1
计算分子量 30 kDa
观测分子量 23 kDa
GenBank蛋白编号BC001114
基因名称 PSMB8
Gene ID (NCBI) 5696
RRIDAB_2882105
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP28062
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).