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PSMB8 Monoclonal antibody

PSMB8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A5B6

Cat no : 66759-1-Ig

Print datasheet

Synonyms

Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1

验证数据展示

  • IHC staining of human lung cancer using 66759-1-Ig

    IHC staining of human lung cancer using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • IHC staining of human lung cancer using 66759-1-Ig

    IHC staining of human lung cancer using 66759-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66759-1-Ig (PSMB8 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMB8 antibody included
  • WB analysis using 66759-1-Ig

    WB analysis using 66759-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66759-1-Ig (PSMB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of human lung cancer using 66759-1-Ig

    IF Staining of human lung cancer using 66759-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 66759-1-Ig

    IF Staining of HepG2 using 66759-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB8 antibody (66759-1-Ig, Clone: 2A5B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, HSC-T6 cells, Raji cells, Ramos cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human oesophagus cancer tissue, mouse colon tissue, mouse stomach tissue, rat colon tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue, human liver cancer tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB8 Ready-To-Use IHC Kit. PSMB8 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66759-1-Ig targets PSMB8 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PSMB8 fusion protein Ag6780 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Synonyms Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC001114
Gene Symbol PSMB8
Gene ID (NCBI) 5696
RRIDAB_2882105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28062
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).

实验方案

Product Specific Protocols
WB protocol for PSMB8 antibody 66759-1-IgDownload protocol
IHC protocol for PSMB8 antibody 66759-1-IgDownload protocol
IF protocol for PSMB8 antibody 66759-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols