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验证数据展示

  • IF Staining of C2C12 using CL594-66759

    IF Staining of C2C12 using CL594-66759

    N/A

  • IF Staining of C2C12 using CL594-66759

    IF Staining of C2C12 using CL594-66759

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200, CL488-phalloidin (blue).

  • IF Staining of human liver cancer using CL594-66759

    IF Staining of human liver cancer using CL594-66759

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66759

    FC experiment of HeLa using CL594-66759

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PSMB8 (CL594-66759, Clone:2A5B6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of human liver cancer using CL594-66759

    IF Staining of human liver cancer using CL594-66759

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

  • IF Staining of HeLa using CL594-66759

    IF Staining of HeLa using CL594-66759

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PSMB8 antibody (CL594-66759, Clone: 2A5B6 ) at dilution of 1:200.

CoraLite®594-conjugated PSMB8 Monoclonal antibody

PSMB8 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL594-66759

Print datasheet

CloneNo. 2A5B6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inC2C12 cells, human liver cancer tissue, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMB8 Ready-To-Use IHC Kit. PSMB8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66759-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66759 targets PSMB8 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen PSMB8 fusion protein Ag6780 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Synonyms Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC001114
Gene Symbol PSMB8
Gene ID (NCBI) 5696
RRIDAB_2920031
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP28062
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).

实验方案

Product Specific Protocols
IF protocol for CL594 PSMB8 antibody CL594-66759Download protocol
Standard Protocols
Click here to view our Standard Protocols