CoraLite®594-conjugated PSMB8 Monoclonal antibody
PSMB8 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2A5B6
验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | C2C12 cells, human liver cancer tissue, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PSMB8 Ready-To-Use IHC Kit. PSMB8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66759-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66759 targets PSMB8 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IF-P, FC (Intra) Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | PSMB8 fusion protein Ag6780 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) |
| Synonyms | Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1 |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC001114 |
| Gene Symbol | PSMB8 |
| Gene ID (NCBI) | 5696 |
| RRID | AB_2920031 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P28062 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 PSMB8 antibody CL594-66759 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
