验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, Y79 cells |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Planning an IHC experiment? We recommend our IHCeasy PSMD13 Ready-To-Use IHC Kit. PSMD13 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
15261-1-AP targets PSMD13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | PSMD13 fusion protein Ag7257 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 |
| 别名 | S11, Rpn9, p40.5, HSPC027, 26S proteasome non-ATPase regulatory subunit 13 |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC001100 |
| 基因名称 | PSMD13 |
| Gene ID (NCBI) | 5719 |
| RRID | AB_2172977 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNM6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMD13 antibody 15261-1-AP | Download protocol |
| IHC protocol for PSMD13 antibody 15261-1-AP | Download protocol |
| IF protocol for PSMD13 antibody 15261-1-AP | Download protocol |
| IP protocol for PSMD13 antibody 15261-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Proteasome gene expression is controlled by coordinated functions of multiple transcription factors | ||
J Neurosci Pharmacological Amelioration of Cone Survival and Vision in a Mouse Model for Leber Congenital Amaurosis.
| ||
J Biol Chem Rescue of enzymatic function for disease-associated RPE65s containing various missense mutations in non-active sites. | ||
J Biochem Temperature sensitive retinoid isomerase activity of RPE65 mutants associated with Leber congenital amaurosis. | ||
bioRxiv Proteasome gene expression is controlled by the coordinated functions of multiple transcription factors |
