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PSMD14/POH1 Recombinant antibody

PSMD14/POH1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242160H6

Cat no : 84767-4-RR

Print datasheet

Synonyms

PSMD14, POH1, PAD1, EC:3.4.19.-, 26S proteasome-associated PAD1 homolog 1

验证数据展示

  • IF Staining of A375 using 84767-4-RR

    IF Staining of A375 using 84767-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using PSMD14 antibody (84767-4-RR, Clone: 242160H6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy PSMD14 Ready-To-Use IHC Kit. PSMD14 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84767-4-RR targets PSMD14/POH1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PSMD14/POH1 fusion protein Ag2694 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 14
Synonyms PSMD14, POH1, PAD1, EC:3.4.19.-, 26S proteasome-associated PAD1 homolog 1
Calculated Molecular Weight 35 kDa
GenBank Accession NumberBC009524
Gene Symbol PSMD14
Gene ID (NCBI) 10213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO00487
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The PSMD14 (POH1, also known as Rpn11/MPR1/S13/CepP1) protein is a metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. PSMD14 is highly expressed in the heart and skeletal muscle. In carcinoma cell lines. down-regulation of PSMD14 by siRNA transfection considerably impacted cell viability, causing cell arrest in the G0-G1 phase, ultimately leading to senescence.

实验方案

Product Specific Protocols
IF protocol for PSMD14/POH1 antibody 84767-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols