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PSMD5 Polyclonal antibody

PSMD5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat No : 11469-1-AP

Print datasheet

Synonyms

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

验证数据展示

  • WB analysis of HeLa using 11469-1-AP

    WB analysis of HeLa using 11469-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11469-1-AP (PSMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11469-1-AP

    IHC staining of human prostate cancer using 11469-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11469-1-AP (PSMD5 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PSMD5 antibody included
  • IP experiment of mouse liver using 11469-1-AP

    IP experiment of mouse liver using 11469-1-AP

    IP result of anti-PSMD5 (IP:11469-1-AP, 4ug; Detection:11469-1-AP 1:1000) with mouse liver tissue lysate 4000 ug.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, human liver tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMD5 Ready-To-Use IHC Kit. PSMD5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11469-1-AP targets PSMD5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PSMD5 fusion protein Ag2026 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Synonyms 26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC014478
Gene Symbol PSMD5
Gene ID (NCBI) 5711
RRIDAB_2170648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16401
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD5, also named as KIAA0072, belongs to the proteasome subunit S5B/HSM3 family. It acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly, it is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a SMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5. (PMID:15489334). This antibody is specific to PSMD5.

实验方案

Product Specific Protocols
WB protocol for PSMD5 antibody 11469-1-APDownload protocol
IHC protocol for PSMD5 antibody 11469-1-APDownload protocol
IP protocol for PSMD5 antibody 11469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols