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PSMD8 Polyclonal antibody

PSMD8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27504-1-AP

Print datasheet

Synonyms

HIP6, HYPF, Nin1p, p31, PSMD8, Rpn12, S14

验证数据展示

  • WB analysis using 27504-1-AP

    WB analysis using 27504-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27504-1-AP (PSMD8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 27504-1-AP

    IF Staining of U2OS using 27504-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PSMD8 antibody (27504-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27504-1-AP targets PSMD8 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen PSMD8 fusion protein Ag26567 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
Synonyms HIP6, HYPF, Nin1p, p31, PSMD8, Rpn12, S14
Calculated Molecular Weight 257 aa, 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC001164
Gene Symbol PSMD8
Gene ID (NCBI) 5714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Proteasome 26S Subunit, Non-ATPase (PSMD) gene family, which includes the PSMD1 to PSMD14, regulate ubiqutinated protein breakdown in circulation as well as tumorigenesis. Studies have shown that PSMD8 interacts with the sperm adhesin AQN1 to limit polyfertilization. PSMD8 was more expressed in normal skeletal muscle, cardiac muscle, and tongue muscle; among malignant tumors, PSMD8 was expressed in ovarian cancer, testicular cancer, glioma, and melanoma(PMID:37349676).

实验方案

Product Specific Protocols
WB protocol for PSMD8 antibody 27504-1-APDownload protocol
IF protocol for PSMD8 antibody 27504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols