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- KD/KO Validated
CoraLite® Plus 488-conjugated PSMD9 Monoclonal antibody
PSMD9 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1H2G1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells, U2OS cells |
For other applications, we recommend the unconjugated version of this antibody, 67338-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67338 targets PSMD9 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | PSMD9 fusion protein Ag25654 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 |
| Synonyms | p27, PSMD9, Rpn4 |
| Calculated Molecular Weight | 27 kDa |
| GenBank Accession Number | BC004213 |
| Gene Symbol | PSMD9 |
| Gene ID (NCBI) | 5715 |
| RRID | AB_2919454 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O00233 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PSMD9 antibody CL488-67338 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
