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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PSME1 Monoclonal antibody

PSME1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F7E2

Cat no : CL488-67928

Print datasheet

Synonyms

REG-alpha, PA28A, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111

验证数据展示

  • FC experiment of HepG2 using CL488-67928

    FC experiment of HepG2 using CL488-67928

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PSME1 (CL488-67928, Clone:1F7E2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSME1 Ready-To-Use IHC Kit. PSME1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67928-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67928 targets PSME1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen PSME1 fusion protein Ag30549 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Synonyms REG-alpha, PA28A, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, IFI5111
Calculated Molecular Weight 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC007503
Gene Symbol PSME1
Gene ID (NCBI) 5720
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ06323
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PSME1 antibody CL488-67928Download protocol
Standard Protocols
Click here to view our Standard Protocols