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验证数据展示

  • WB analysis using 67629-1-Ig

    WB analysis using 67629-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67629-1-Ig

    WB analysis using 67629-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67629-1-Ig

    IHC staining of human liver cancer using 67629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSME3 antibody included
  • IHC staining of human liver cancer using 67629-1-Ig

    IHC staining of human liver cancer using 67629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSME3 antibody included

PSME3 Monoclonal antibody

PSME3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67629-1-Ig

产品说明书

CloneNo. 2A8E8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat

应用

WB, IHC, ELISA

别名

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSME3 Ready-To-Use IHC Kit. PSME3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67629-1-Ig targets PSME3 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Rat
免疫原 PSME3 fusion protein Ag6716 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
别名 Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA
计算分子量 30 kDa
观测分子量 32 kDa
GenBank蛋白编号BC001423
基因名称 PSME3
Gene ID (NCBI) 10197
RRIDAB_2882830
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61289
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

实验方案

Product Specific Protocols
WB protocol for PSME3 antibody 67629-1-IgDownload protocol
IHC protocol for PSME3 antibody 67629-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols