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PSME3 Monoclonal antibody

PSME3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A8E8

Cat no : 67629-1-Ig

Print datasheet

Synonyms

Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA

验证数据展示

  • WB analysis using 67629-1-Ig

    WB analysis using 67629-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67629-1-Ig

    WB analysis using 67629-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67629-1-Ig (PSME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67629-1-Ig

    IHC staining of human liver cancer using 67629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSME3 antibody included
  • IHC staining of human liver cancer using 67629-1-Ig

    IHC staining of human liver cancer using 67629-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67629-1-Ig (PSME3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSME3 antibody included

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSME3 Ready-To-Use IHC Kit. PSME3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67629-1-Ig targets PSME3 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Rat
Immunogen PSME3 fusion protein Ag6716 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
Synonyms Ki, Ki nuclear autoantigen, PA28 gamma, PA28G, PA28gamma, PSME3, REG GAMMA
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC001423
Gene Symbol PSME3
Gene ID (NCBI) 10197
RRIDAB_2882830
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61289
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

实验方案

Product Specific Protocols
WB protocol for PSME3 antibody 67629-1-IgDownload protocol
IHC protocol for PSME3 antibody 67629-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols