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PSMF1 Polyclonal antibody

PSMF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 12941-1-AP

Print datasheet

Synonyms

hPI31, PI31, PSMF1

验证数据展示

  • WB analysis using 12941-1-AP

    WB analysis using 12941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12941-1-AP

    WB analysis of HEK-293 using 12941-1-AP

    WB result of PSMF1 antibody (12941-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMF1 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHEK-293 cells, human kidney tissue, human heart tissue, Jurkat cells, Neuro-2a cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12941-1-AP targets PSMF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PSMF1 fusion protein Ag4011 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
Synonyms hPI31, PI31, PSMF1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC029836
Gene Symbol PSMF1
Gene ID (NCBI) 9491
RRIDAB_2268855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92530
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMF1 is also named as PI31(proteasome (prosome, macropain) inhibitor subunit 1) and belongs to proteasome inhibitor PI31 family. PI31, which localizes at the nuclear envelope/endoplasmic reticulum membrane, acts as a selective modulator of the proteasome-mediated steps in MHC class I antigen processing(PMID:18495667). PI31 does not inhibit cell-surface transport of glycoproteins in general and it may serve to control immunoproteasome formation and may thereby maintain an intracellular balance between constitutive and immunoproteasomes(PMID:12374861).

实验方案

Product Specific Protocols
WB protocol for PSMF1 antibody 12941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Biol

Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.

Authors - Hongli An
View Article