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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HepG2 using 66921-1-Ig (same clone as 66921-1-PBS)

    FC experiment of HepG2 using 66921-1-Ig (same clone as 66921-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PTGER4 (66921-1-Ig, Clone:4A2A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • WB analysis of pig brain using 66921-1-Ig (same clone as 66921-1-PBS)

    WB analysis of pig brain using 66921-1-Ig (same clone as 66921-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66921-1-Ig (PTGER4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

    IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

    IHC staining of human prostate hyperplasia using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66921-1-Ig (PTGER4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    IF Staining of mouse brain using 66921-1-Ig (same clone as 66921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PTGER4 antibody (66921-1-Ig, Clone: 4A2A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66921-1-PBS in a different storage buffer formulation.

PTGER4 Monoclonal antibody, PBS Only

PTGER4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66921-1-PBS

产品说明书

CloneNo. 4A2A12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

EP4R, EP4, 4A2A12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66921-1-PBS targets PTGER4 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PTGER4 fusion protein Ag19262 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 prostaglandin E receptor 4 (subtype EP4)
别名 EP4R, EP4, 4A2A12
计算分子量 488 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC101534
基因名称 PTGER4
Gene ID (NCBI) 5734
RRIDAB_2882248
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35408
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.