PTGER4 Monoclonal antibody
PTGER4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
4A2A12
验证数据展示
经过测试的应用
Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue, Jurkat cells, K-562 cells |
Positive IHC detected in | human prostate hyperplasia tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
66921-1-Ig targets PTGER4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse |
Immunogen | PTGER4 fusion protein Ag19262 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | prostaglandin E receptor 4 (subtype EP4) |
Synonyms | EP4R, EP4, 4A2A12 |
Calculated Molecular Weight | 488 aa, 53 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC101534 |
Gene Symbol | PTGER4 |
Gene ID (NCBI) | 5734 |
RRID | AB_2882248 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35408 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PTGER4 antibody 66921-1-Ig | Download protocol |
IHC protocol for PTGER4 antibody 66921-1-Ig | Download protocol |
IF protocol for PTGER4 antibody 66921-1-Ig | Download protocol |
FC protocol for PTGER4 antibody 66921-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther m6A demethylase ALKBH5 is required for antibacterial innate defense by intrinsic motivation of neutrophil migration. | ||
J Mol Med (Berl) Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation | ||
Nat Commun Post-resolution macrophages shape long-term tissue immunity and integrity in a mouse model of pneumococcal pneumonia |