验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue, Jurkat cells, K-562 cells |
| Positive IHC detected in | human prostate hyperplasia tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
66921-1-Ig targets PTGER4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Immunogen | PTGER4 fusion protein Ag19262 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | prostaglandin E receptor 4 (subtype EP4) |
| Synonyms | EP4R, EP4, 4A2A12 |
| Calculated Molecular Weight | 488 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC101534 |
| Gene Symbol | PTGER4 |
| Gene ID (NCBI) | 5734 |
| RRID | AB_2882248 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35408 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTGER4 antibody 66921-1-Ig | Download protocol |
| IHC protocol for PTGER4 antibody 66921-1-Ig | Download protocol |
| IF protocol for PTGER4 antibody 66921-1-Ig | Download protocol |
| FC protocol for PTGER4 antibody 66921-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther m6A demethylase ALKBH5 is required for antibacterial innate defense by intrinsic motivation of neutrophil migration. | ||
J Mol Med (Berl) Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation | ||
Nat Commun Post-resolution macrophages shape long-term tissue immunity and integrity in a mouse model of pneumococcal pneumonia | ||
Cell Metab m6A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1 |
