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CoraLite® Plus 488-conjugated PTGER4 Monoclonal antibody

PTGER4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A2A12

Cat No : CL488-66921

Print datasheet

Synonyms

EP4, EP4R, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor, PTGER2, PTGER4

验证数据展示

  • FC experiment of HepG2 using CL488-66921

    FC experiment of HepG2 using CL488-66921

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PTGER4 (CL488-66921, Clone:4A2A12) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66921-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66921 targets PTGER4 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen PTGER4 fusion protein Ag19262 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name prostaglandin E receptor 4 (subtype EP4)
Synonyms EP4, EP4R, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor, PTGER2, PTGER4
Calculated Molecular Weight 488 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC101534
Gene Symbol PTGER4
Gene ID (NCBI) 5734
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35408
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PTGER4 antibody CL488-66921Download protocol
Standard Protocols
Click here to view our Standard Protocols