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验证数据展示

  • IF Staining of HepG2 using CL488-67736

    IF Staining of HepG2 using CL488-67736

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PTGES3 antibody (CL488-67736, Clone: 3C11D11 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67736

    FC experiment of HepG2 using CL488-67736

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PTGES3 (CL488-67736, Clone:3C11D11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated PTGES3 Monoclonal antibody

PTGES3 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67736

Print datasheet

CloneNo. 3C11D11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

P23, Hsp90 co-chaperone, Hsp90 co chaperone, EC:5.3.99.3, cPGES

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTGES3 Ready-To-Use IHC Kit. PTGES3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67736-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67736 targets PTGES3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PTGES3 fusion protein Ag7870 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name prostaglandin E synthase 3 (cytosolic)
Synonyms P23, Hsp90 co-chaperone, Hsp90 co chaperone, EC:5.3.99.3, cPGES
Calculated Molecular Weight 19 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC003005
Gene Symbol PTGES3
Gene ID (NCBI) 10728
RRIDAB_2923784
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15185
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P23, encoded by the gene PTGES3, plays a key role in glucocorticoid signaling and was increased at the mRNA level in the DLPFC in individuals with schizophrenia (PMID: 24345775). In the GR heterocomplex in vitro, p23 is an obligatory co-factor19 and is the limiting component of the complex44, functioning to stabilize the interaction of the complex with GR in the cytoplasm. Paradoxically, p23 also acts in the nucleus to inhibit GR-mediated gene transcription and disassemble GR transcriptional machinery in the nucleus (PMID: 12077419). In vivo, p23 is critical for appropriate glucocorticoid responsiveness (PMID: 17000766).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PTGES3 antibody CL488-67736Download protocol
FC protocol for CL Plus 488 PTGES3 antibody CL488-67736Download protocol
Standard Protocols
Click here to view our Standard Protocols