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验证数据展示

  • WB analysis of L02 using 67346-1-Ig

    WB analysis of L02 using 67346-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67346-1-Ig

    IF Staining of HeLa using 67346-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67346-1-Ig (PTGS1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

COX-1/Cyclooxygenase-1 Monoclonal antibody

COX-1/Cyclooxygenase-1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67346-1-Ig

产品说明书

CloneNo. 1F12G7

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, K-562 cells, A431 cells, Human peripheral blood leukocyte
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67346-1-Ig targets COX-1/Cyclooxygenase-1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 COX-1/Cyclooxygenase-1 fusion protein Ag28280 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
别名 PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1
计算分子量 599 aa, 69 kDa
观测分子量 60-72 kDa
GenBank蛋白编号BC029840
基因名称 PTGS1
Gene ID (NCBI) 5742
RRIDAB_2882604
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23219
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

实验方案

Product Specific Protocols
WB protocol for COX-1/Cyclooxygenase-1 antibody 67346-1-IgDownload protocol
IF protocol for COX-1/Cyclooxygenase-1 antibody 67346-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols