• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

COX-1/Cyclooxygenase-1 Monoclonal antibody

COX-1/Cyclooxygenase-1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F12G7

Cat no : 67346-1-Ig

Print datasheet

Synonyms

PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1

验证数据展示

  • WB analysis of L02 using 67346-1-Ig

    WB analysis of L02 using 67346-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67346-1-Ig (PTGS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67346-1-Ig

    IF Staining of HeLa using 67346-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67346-1-Ig (PTGS1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, K-562 cells, A431 cells, Human peripheral blood leukocyte
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67346-1-Ig targets COX-1/Cyclooxygenase-1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen COX-1/Cyclooxygenase-1 fusion protein Ag28280 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Synonyms PGH1, PTGS1, PGHS-1, EC:1.14.99.1, Cyclooxygenase-1
Calculated Molecular Weight 599 aa, 69 kDa
Observed Molecular Weight 60-72 kDa
GenBank Accession NumberBC029840
Gene Symbol PTGS1
Gene ID (NCBI) 5742
RRIDAB_2882604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTGS1(Prostaglandin G/H synthase 1) belongs to the prostaglandin G/H synthase family and catalyzes the conversion of arachidonic acid to prostaglandin H2, which is subsequently metabolized to various biologically active prostaglandins. It is also named as COX1(Cyclooxygenase-1). There is no PTGS1 expression in fetal samples (prostate, seminal vesicles, or ejaculatory ducts), and only minimal expression in adult tissues (PMID: 10999846). PTGS1 has some isoforms with MW of 56-72 kD and PTGS1 can form homodimer.

实验方案

Product Specific Protocols
WB protocol for COX-1/Cyclooxygenase-1 antibody 67346-1-IgDownload protocol
IF protocol for COX-1/Cyclooxygenase-1 antibody 67346-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols