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PTK2B Monoclonal antibody, PBS Only

PTK2B Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10G5

Cat no : 67141-1-PBS

Print datasheet

Synonyms

PYK2, Calcium-dependent tyrosine kinase, CAK-beta, CAKB, CAK beta

验证数据展示

  • WB analysis using 67141-1-Ig (same clone as 67141-1-PBS)

    WB analysis using 67141-1-Ig (same clone as 67141-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67141-1-Ig (PTK2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

    IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67141-1-Ig (PTK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTK2B antibody included
  • IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

    IHC staining of mouse brain using 67141-1-Ig (same clone as 67141-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67141-1-Ig (PTK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67141-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTK2B antibody included
Planning an IHC experiment? We recommend our IHCeasy PTK2B Ready-To-Use IHC Kit. PTK2B primary antibody included.

产品信息

67141-1-PBS targets PTK2B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen PTK2B fusion protein Ag11487 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name PTK2B protein tyrosine kinase 2 beta
Synonyms PYK2, Calcium-dependent tyrosine kinase, CAK-beta, CAKB, CAK beta
Calculated Molecular Weight 1009 aa, 116 kDa
Observed Molecular Weight 112-115 kDa
GenBank Accession NumberBC042599
Gene Symbol PTK2B
Gene ID (NCBI) 2185
RRIDAB_2882440
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14289
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.