验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, mouse brain tissue, K-562 cells, Ramos cells, pig brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
67141-1-Ig targets PYK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat, pig |
文献引用反应性 | human |
免疫原 | PYK2 fusion protein Ag11487 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | PTK2B protein tyrosine kinase 2 beta |
别名 | PTK2B, 2A10G5, CADTK, CAK beta, CAKB |
计算分子量 | 1009 aa, 116 kDa |
观测分子量 | 112-115 kDa |
GenBank蛋白编号 | BC042599 |
基因名称 | PTK2B |
Gene ID (NCBI) | 2185 |
RRID | AB_2882440 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q14289 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PYK2 antibody 67141-1-Ig | Download protocol |
IHC protocol for PYK2 antibody 67141-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |