PYK2 Monoclonal antibody

PYK2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67141-1-Ig

产品说明书

CloneNo. 2A10G5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

PTK2B, 2A10G5, CADTK, CAK beta, CAKB

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, mouse brain tissue, K-562 cells, Ramos cells, pig brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTK2B Ready-To-Use IHC Kit. PTK2B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67141-1-Ig targets PYK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原 PYK2 fusion protein Ag11487 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PTK2B protein tyrosine kinase 2 beta
别名 PTK2B, 2A10G5, CADTK, CAK beta, CAKB
计算分子量 1009 aa, 116 kDa
观测分子量 112-115 kDa
GenBank蛋白编号BC042599
基因名称 PTK2B
Gene ID (NCBI) 2185
RRIDAB_2882440
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14289
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.

实验方案

Product Specific Protocols
WB protocol for PYK2 antibody 67141-1-IgDownload protocol
IHC protocol for PYK2 antibody 67141-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Discovery of electrophilic degraders that exploit SNAr chemistry

Authors - Zhe Zhuang
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