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验证数据展示

  • FC experiment of Jurkat using CL488-67141

    FC experiment of Jurkat using CL488-67141

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PTK2B (CL488-67141, Clone:2A10G5) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated PTK2B Monoclonal antibody

PTK2B Monoclonal Antibody for FC (Intra)
Cat No. CL488-67141

产品说明书

CloneNo. 2A10G5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

FC (Intra)

别名

PYK2, Calcium-dependent tyrosine kinase, CAK-beta, CAKB, CAK beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PTK2B Ready-To-Use IHC Kit. PTK2B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67141-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67141 targets PTK2B in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PTK2B fusion protein Ag11487 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PTK2B protein tyrosine kinase 2 beta
别名 PYK2, Calcium-dependent tyrosine kinase, CAK-beta, CAKB, CAK beta
计算分子量 1009 aa, 116 kDa
观测分子量 112-115 kDa
GenBank蛋白编号BC042599
基因名称 PTK2B
Gene ID (NCBI) 2185
RRIDAB_3084314
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14289
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PTK2B antibody CL488-67141Download protocol
Standard Protocols
Click here to view our Standard Protocols