CoraLite® Plus 488-conjugated PTK2B Monoclonal antibody
PTK2B Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A10G5
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 67141-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67141 targets PTK2B in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat, pig |
Immunogen | PTK2B fusion protein Ag11487 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | PTK2B protein tyrosine kinase 2 beta |
Synonyms | PYK2, Calcium-dependent tyrosine kinase, CAK-beta, CAKB, CAK beta |
Calculated Molecular Weight | 1009 aa, 116 kDa |
Observed Molecular Weight | 112-115 kDa |
GenBank Accession Number | BC042599 |
Gene Symbol | PTK2B |
Gene ID (NCBI) | 2185 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q14289 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 488 PTK2B antibody CL488-67141 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |