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验证数据展示

  • WB analysis using 67584-1-Ig

    WB analysis using 67584-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67584-1-Ig

    IHC staining of human lung cancer using 67584-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTP4A1 antibody included
  • IHC staining of human lung cancer using 67584-1-Ig

    IHC staining of human lung cancer using 67584-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTP4A1 antibody included
  • IF Staining of HeLa using 67584-1-Ig

    IF Staining of HeLa using 67584-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTP4A1 antibody (67584-1-Ig, Clone: 2C9E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

PTP4A1 Monoclonal antibody

PTP4A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67584-1-Ig

产品说明书

CloneNo. 2C9E10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PRL1, PTP4A1/PRL1, Protein-tyrosine phosphatase of regenerating liver 1, Protein-tyrosine phosphatase 4a1, PRL-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PTP4A1 Ready-To-Use IHC Kit. PTP4A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67584-1-Ig targets PTP4A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PTP4A1 fusion protein Ag14436 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase type IVA, member 1
别名 PRL1, PTP4A1/PRL1, Protein-tyrosine phosphatase of regenerating liver 1, Protein-tyrosine phosphatase 4a1, PRL-1
计算分子量 20 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC023975
基因名称 PTP4A1
Gene ID (NCBI) 7803
RRIDAB_2923632
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93096
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).

实验方案

Product Specific Protocols
WB protocol for PTP4A1 antibody 67584-1-IgDownload protocol
IHC protocol for PTP4A1 antibody 67584-1-IgDownload protocol
IF protocol for PTP4A1 antibody 67584-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols