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验证数据展示

  • WB analysis using 67584-1-Ig (same clone as 67584-1-PBS)

    WB analysis using 67584-1-Ig (same clone as 67584-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

    IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTP4A1 antibody included
  • IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

    IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTP4A1 antibody included
  • IF Staining of HeLa using 67584-1-Ig (same clone as 67584-1-PBS)

    IF Staining of HeLa using 67584-1-Ig (same clone as 67584-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTP4A1 antibody (67584-1-Ig, Clone: 2C9E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

PTP4A1 Monoclonal antibody, PBS Only

PTP4A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67584-1-PBS

产品说明书

CloneNo. 2C9E10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PRL1, PTP4A1/PRL1, Protein-tyrosine phosphatase of regenerating liver 1, Protein-tyrosine phosphatase 4a1, PRL-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PTP4A1 Ready-To-Use IHC Kit. PTP4A1 primary antibody included.

产品信息

67584-1-PBS targets PTP4A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PTP4A1 fusion protein Ag14436 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase type IVA, member 1
别名 PRL1, PTP4A1/PRL1, Protein-tyrosine phosphatase of regenerating liver 1, Protein-tyrosine phosphatase 4a1, PRL-1
计算分子量 20 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC023975
基因名称 PTP4A1
Gene ID (NCBI) 7803
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93096
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).