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PTPIP51 Monoclonal antibody

PTPIP51 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G11E2

Cat no : 68582-1-Ig

Print datasheet

Synonyms

Cerebral protein 10, FAM82A2, FAM82C, FLJ10579, hRMD 3, Protein FAM82A2, Protein FAM82C, PTPIP51, RMD 3, RMD3, TCPTP interacting protein 51, UNQ3122/PRO10274

验证数据展示

  • WB analysis using 68582-1-Ig

    WB analysis using 68582-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68582-1-Ig (PTPIP51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, HepG2 cells, HuH-7 cells, HT-29 cells, HEK-293 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68582-1-Ig targets PTPIP51 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen PTPIP51 fusion protein Ag15135 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name family with sequence similarity 82, member A2
Synonyms Cerebral protein 10, FAM82A2, FAM82C, FLJ10579, hRMD 3, Protein FAM82A2, Protein FAM82C, PTPIP51, RMD 3, RMD3, TCPTP interacting protein 51, UNQ3122/PRO10274
Calculated Molecular Weight 470 aa, 52 kDa
Observed Molecular Weight52-60 kDa
GenBank Accession NumberBC008970
Gene Symbol PTPIP51
Gene ID (NCBI) 55177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96TC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618).The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).

实验方案

Product Specific Protocols
WB protocol for PTPIP51 antibody 68582-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols