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SHP2 Monoclonal antibody, PBS Only

SHP2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3F8A8

Cat no : 66795-1-PBS

Print datasheet

Synonyms

PTPN11, NS1, NS 1, EC:3.1.3.48, CFC

验证数据展示

  • WB analysis using 66795-1-Ig (same clone as 66795-1-PBS)

    WB analysis using 66795-1-Ig (same clone as 66795-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66795-1-Ig (PTPN11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPN11 antibody included
  • FC experiment of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    FC experiment of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PTPN11 (66795-1-Ig, Clone:3F8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (66795-1-Ig, Clone: 3F8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.

产品信息

66795-1-PBS targets SHP2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SHP2 fusion protein Ag13649 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 11
Synonyms PTPN11, NS1, NS 1, EC:3.1.3.48, CFC
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06124
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.