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PTPN11 Monoclonal antibody, PBS Only

PTPN11 Monoclonal Antibody for WB,IHC,IF,FC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

3F8A8

Cat No : 66795-1-PBS

Print datasheet

Synonyms

BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2

验证数据展示

  • WB analysis using 66795-1-Ig (same clone as 66795-1-PBS)

    WB analysis using 66795-1-Ig (same clone as 66795-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66795-1-Ig (PTPN11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    IHC staining of mouse testis using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66795-1-Ig (PTPN11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPN11 antibody included
  • FC experiment of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    FC experiment of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PTPN11 (66795-1-Ig, Clone:3F8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    IF Staining of MCF-7 using 66795-1-Ig (same clone as 66795-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66795-1-Ig (PTPN11 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66795-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.

产品信息

66795-1-PBS targets PTPN11 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IHC,IF,FC,ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PTPN11 fusion protein Ag13649 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 11
Synonyms BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06124
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.