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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of A431 using 83851-6-RR (same clone as 83851-6-PBS)

    IF Staining of A431 using 83851-6-RR (same clone as 83851-6-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (83851-6-RR, Clone: 240983C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 83851-6-RR (same clone as 83851-6-PBS)

    IF Staining of U-251 using 83851-6-RR (same clone as 83851-6-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PTPN12 antibody (83851-6-RR, Clone: 240983C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

  • WB analysis using 83851-6-RR (same clone as 83851-6-PBS)

    WB analysis using 83851-6-RR (same clone as 83851-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83851-6-RR (PTPN12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83851-6-RR (same clone as 83851-6-PBS)

    IHC staining of human ovary cancer using 83851-6-RR (same clone as 83851-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83851-6-RR (PTPN12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83851-6-RR

    Affinity and Kinetic Characterization of 83851-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83851-6-RR against Human PTPN12 were performed. The affinity constant is 48.4 pM.

PTPN12 Recombinant antibody, PBS Only

PTPN12 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83851-6-PBS

Print datasheet

CloneNo. 240983C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PTPG1, PTP PEST, 240983C9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83851-6-PBS targets PTPN12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PTPN12 fusion protein Ag35382 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 12
Synonyms PTPG1, PTP PEST, 240983C9
Calculated Molecular Weight88kDa
Observed Molecular Weight110-125 kDa
GenBank Accession NumberNM_002835.3
Gene Symbol PTPN12
Gene ID (NCBI) 5782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ05209
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.