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验证数据展示

  • WB analysis using 67744-1-Ig (same clone as 67744-1-PBS)

    WB analysis using 67744-1-Ig (same clone as 67744-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67744-1-Ig (PTPN14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

    IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

    IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67744-1-Ig (same clone as 67744-1-PBS)

    IF Staining of HeLa using 67744-1-Ig (same clone as 67744-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTPN14 antibody (67744-1-Ig, Clone: 1B8C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

PTPN14 Monoclonal antibody, PBS Only

PTPN14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67744-1-PBS

产品说明书

CloneNo. 1B8C8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PTP36, PTP 36, Protein-tyrosine phosphatase pez, PEZ, EC:3.1.3.48

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67744-1-PBS targets PTPN14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PTPN14 fusion protein Ag12679 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 14
别名 PTP36, PTP 36, Protein-tyrosine phosphatase pez, PEZ, EC:3.1.3.48
计算分子量 1187 aa, 135 kDa
观测分子量150 kDa
GenBank蛋白编号BC101754
基因名称 PTPN14
Gene ID (NCBI) 5784
RRIDAB_2918513
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15678
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).