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PTPN14 Monoclonal antibody

PTPN14 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1B8C8

Cat no : 67744-1-Ig

Print datasheet

Synonyms

PEZ, PTP36, PTPN14

验证数据展示

  • WB analysis using 67744-1-Ig

    WB analysis using 67744-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67744-1-Ig (PTPN14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 67744-1-Ig

    IHC staining of human placenta using 67744-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 67744-1-Ig

    IHC staining of human placenta using 67744-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU2OS cells, A549 cells, HEK-293 cells, Hela cells, K-562 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67744-1-Ig targets PTPN14 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen PTPN14 fusion protein Ag12679 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 14
Synonyms PEZ, PTP36, PTPN14
Calculated Molecular Weight 1187 aa, 135 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC101754
Gene Symbol PTPN14
Gene ID (NCBI) 5784
RRIDAB_2918513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15678
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

实验方案

Product Specific Protocols
WB protocol for PTPN14 antibody 67744-1-IgDownload protocol
IHC protocol for PTPN14 antibody 67744-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Biomed Pharmacother

XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma

Authors - Zengbin Wang
View Article