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验证数据展示

  • IF Staining of HeLa using CL647-67744

    IF Staining of HeLa using CL647-67744

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 PTPN14 antibody (CL647-67744, Clone: 1B8C8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL647-67744

    FC experiment of HeLa using CL647-67744

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human PTPN14 (CL647-67744, Clone:1B8C8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated PTPN14 Monoclonal antibody

PTPN14 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-67744

Print datasheet

CloneNo. 1B8C8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

PTP36, PTP 36, Protein-tyrosine phosphatase pez, PEZ, EC:3.1.3.48

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67744-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67744 targets PTPN14 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen PTPN14 fusion protein Ag12679 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 14
Synonyms PTP36, PTP 36, Protein-tyrosine phosphatase pez, PEZ, EC:3.1.3.48
Calculated Molecular Weight 1187 aa, 135 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC101754
Gene Symbol PTPN14
Gene ID (NCBI) 5784
RRIDAB_2935085
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15678
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 PTPN14 antibody CL647-67744Download protocol
FC protocol for CL Plus 647 PTPN14 antibody CL647-67744Download protocol
Standard Protocols
Click here to view our Standard Protocols