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验证数据展示

  • WB analysis using 12575-1-AP

    WB analysis using 12575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12575-1-AP

    IP experiment of HEK-293 using 12575-1-AP

    IP result of anti-PTTG1IP (IP:12575-1-AP, 4ug; Detection:12575-1-AP 1:300) with HEK-293 cells lysate 2600 ug.

  • IHC staining of human testis using 12575-1-AP

    IHC staining of human testis using 12575-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 12575-1-AP

    IHC staining of human testis using 12575-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 12575-1-AP

    IF Staining of HeLa using 12575-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTTG1IP antibody (12575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

PTTG1IP Polyclonal antibody

PTTG1IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12575-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PTTG-binding factor, Pituitary tumor-transforming gene protein-binding factor, Pituitary tumor-transforming gene 1 protein-interacting protein, C21orf3, C21orf1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human colon tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12575-1-AP targets PTTG1IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 PTTG1IP fusion protein Ag3250 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pituitary tumor-transforming 1 interacting protein
别名 PTTG-binding factor, Pituitary tumor-transforming gene protein-binding factor, Pituitary tumor-transforming gene 1 protein-interacting protein, C21orf3, C21orf1
计算分子量 180 aa, 20 kDa
观测分子量 26 kDa
GenBank蛋白编号BC012858
基因名称 PTTG1IP
Gene ID (NCBI) 754
RRIDAB_10644145
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53801
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTTG1IP antibody 12575-1-APDownload protocol
IHC protocol for PTTG1IP antibody 12575-1-APDownload protocol
IF protocol for PTTG1IP antibody 12575-1-APDownload protocol
IP protocol for PTTG1IP antibody 12575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Horm Metab Res

MicroRNA-524-5p Functions as a Tumor Suppressor in a Human Pituitary Tumor-Derived Cell Line.

Authors - Wang Zhen
View Article
humanIHC

Oncol Lett

Expression of proteins associated with epithelial-mesenchymal transition in esophageal squamous cell carcinoma.

Authors - Tian Zhang
View Article
humanWB

Pharmacol Res

PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma

Authors - Wenfang Bao
View Article