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PURA Monoclonal antibody
PURA Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C6G4
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, pig brain tissue, rat brain tissue, mouse brain tissue, L02 cells, LNCaP cells, human placenta tissue, rat cerebellum tissue, mouse cerebellum tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67305-1-Ig targets PURA in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, mouse, rat, pig |
| Immunogen | PURA fusion protein Ag12164 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | purine-rich element binding protein A |
| Synonyms | PUR ALPHA, PUR1, PURA, PURALPHA |
| Calculated Molecular Weight | 322 aa, 35 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC036087 |
| Gene Symbol | PURA |
| Gene ID (NCBI) | 5813 |
| RRID | AB_2882567 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00577 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PURA antibody 67305-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
