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- KD/KO Validated
CoraLite® Plus 488-conjugated PURA Monoclonal antibody
PURA Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C6G4
验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67305-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67305 targets PURA in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | PURA fusion protein Ag12164 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | purine-rich element binding protein A |
| Synonyms | Transcriptional activator protein Pur-alpha, Purine-rich single-stranded DNA-binding protein alpha, PURALPHA, PUR1, PUR ALPHA |
| Calculated Molecular Weight | 322 aa, 35 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC036087 |
| Gene Symbol | PURA |
| Gene ID (NCBI) | 5813 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00577 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 PURA antibody CL488-67305 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
