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  • Featured Product
  • KD/KO Validated

PYCR1 Polyclonal antibody

PYCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13108-1-AP

Print datasheet

Synonyms

PIG45, P5CR 1, P5CR, P5C reductase 1, P5C

验证数据展示

  • WB analysis using 13108-1-AP

    WB analysis using 13108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa cells using 13108-1-AP

    WB analysis of HeLa cells using 13108-1-AP

    WB result of PYCR1 antibody (13108-1-AP, 1:5000) with si-Control and si-PYCR1 transfected HeLa cells.

  • IP experiment of mouse brain using 13108-1-AP

    IP experiment of mouse brain using 13108-1-AP

    IP result of anti-PYCR1 (IP:13108-1-AP, 3ug; Detection:13108-1-AP 1:700) with mouse brain tissue lysate 4000ug.

  • IHC staining of human prostate cancer using 13108-1-AP

    IHC staining of human prostate cancer using 13108-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PYCR1 antibody included
  • IHC staining of human prostate cancer using 13108-1-AP

    IHC staining of human prostate cancer using 13108-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PYCR1 antibody included
  • IF Staining of HepG2 using 13108-1-AP

    IF Staining of HepG2 using 13108-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PYCR1 antibody (13108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, human brain tissue, mouse embryo tissue, mouse liver tissue, NIH/3T3 cells, HT-1080 cells，mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PYCR1 Ready-To-Use IHC Kit. PYCR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13108-1-AP targets PYCR1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Immunogen PYCR1 fusion protein Ag3764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyrroline-5-carboxylate reductase 1
Synonyms PIG45, P5CR 1, P5CR, P5C reductase 1, P5C
Calculated Molecular Weight 319 aa, 33.8 kDa
Observed Molecular Weight 33 kDa, 35 kDa
GenBank Accession NumberBC022244
Gene Symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_2174878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32322
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

实验方案

Product Specific Protocols
WB protocol for PYCR1 antibody 13108-1-APDownload protocol
IHC protocol for PYCR1 antibody 13108-1-APDownload protocol
IF protocol for PYCR1 antibody 13108-1-APDownload protocol
IP protocol for PYCR1 antibody 13108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Nature

Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.

Authors - Fabricio Loayza-Puch
  • KO Validated
View Article
humanWB

Nature

Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.

Authors - Possemato Richard R
View Article
zebrafishWB,IF

Nat Genet

Mutations in PYCR1 cause cutis laxa with progeroid features.

Authors - Reversade Bruno B
View Article
mouseWB

Cell Metab

Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.

Authors - Sukru Anil Dogan
View Article
humanIHC

J Hepatol

Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.

Authors - Zhaobing Ding
View Article
human,mouseWB,IF

Neuron

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Authors - Nathalie Escande-Beillard
View Article