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PYCR1 Polyclonal antibody

PYCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13108-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

PIG45, P5CR 1, P5CR, P5C reductase 1, P5C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, human brain tissue, mouse embryo tissue, mouse liver tissue, NIH/3T3 cells, HT-1080 cells,mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PYCR1 Ready-To-Use IHC Kit. PYCR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13108-1-AP targets PYCR1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, zebrafish
免疫原 PYCR1 fusion protein Ag3764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase 1
别名 PIG45, P5CR 1, P5CR, P5C reductase 1, P5C
计算分子量 319 aa, 33.8 kDa
观测分子量 33 kDa, 35 kDa
GenBank蛋白编号BC022244
基因名称 PYCR1
Gene ID (NCBI) 5831
RRIDAB_2174878
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

实验方案

Product Specific Protocols
WB protocol for PYCR1 antibody 13108-1-APDownload protocol
IHC protocol for PYCR1 antibody 13108-1-APDownload protocol
IF protocol for PYCR1 antibody 13108-1-APDownload protocol
IP protocol for PYCR1 antibody 13108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Nature

Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.

Authors - Fabricio Loayza-Puch
  • KO Validated
humanWB

Nature

Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.

Authors - Possemato Richard R
zebrafishWB,IF

Nat Genet

Mutations in PYCR1 cause cutis laxa with progeroid features.

Authors - Reversade Bruno B
mouseWB

Cell Metab

Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.

Authors - Sukru Anil Dogan
humanIHC

J Hepatol

Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.

Authors - Zhaobing Ding
human,mouseWB,IF

Neuron

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Authors - Nathalie Escande-Beillard
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