IHCeasy PYCR1 Ready-To-Use IHC Kit
PYCR1 Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse
Sample Type
FFPE tissue
Cat No : KHC0833
Synonyms
P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1
Validation Data Gallery
Product Information
KHC0833 is a ready-to-use IHC kit for staining of PYCR1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy PYCR1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1 |