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验证数据展示

  • WB analysis of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    WB analysis of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    IP experiment of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    IP result of anti-PYCR1 (IP:22150-1-AP, 4ug; Detection:22150-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    IF Staining of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22150-1-AP (PYCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PYCR1 Polyclonal antibody, PBS Only

PYCR1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 22150-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PYCR1 Ready-To-Use IHC Kit. PYCR1 primary antibody included.

产品信息

22150-1-PBS targets PYCR1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PYCR1 fusion protein Ag17745 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase 1
别名
计算分子量 319 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC022244
基因名称 PYCR1
Gene ID (NCBI) 5831
RRIDAB_11182280
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32322
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody may have cross reaction to PYCR2 due to the high homology.